Rodent malaria line

Plasmodium yoelii nigeriensis N67

Type
Rodent malaria line
Description
P.y. nigeriensis N67, derived from single oocyst infection
Subclone of
isolate N67
Country
Nigeria
History
The N67 strain of P. yoelii nigeriensis was isolated in Nigeria in 1967 and maintained at the London School of Hygiene and Tropical Medicine, London. Sporozite-transmitted uncloned isolate received Edinburgh, 14.8.68. Stabilates made 11/12/09
UK Biosafety level
1
Availability
Available.
Comments
likely to be clonal
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium chabaudi subsp. EF clones

Type
Rodent malaria line
Description
Plasmodium chabaudi subsp. EF clones, 6EF and 7EF, after mosquito transmission.
Subclone of
isolate Biboto 37
Country
Cameroon
History
Original Biboto 37 isolate from T. rutilans in Cameroon in August 1973 made by J..M. Bafort. Passaged x3 and mosquito. Sent to Edinburgh 21.10.1977 by Marc Wery. 1EF1 passaged x3 through mice, cloning; deep froze stabilates, 6EF4, 29/11/77, 7EF1, 25/11/77.
UK Biosafety level
1
Availability
Available.
Links
Molecular evolution and phylogenetics of rodent malaria parasites
References

Killick-Kendrick R, Peters W. Rodent Malaria. London: Academic Press. pp. 213-245, 1978. Carter R, Walliker D. New observations on the malaria parasites of rodents of the Central African Republic - Plasmodium vinckei petteri subsp. nov. and Plasmodium chabaudi Landau, 1965. Ann. Trop Med. Parasitol. 69: 187-196, 1975.
Lainson F.A.. Observations on the morphology and electrophoretic variation of enzymes of the rodent malaria parasites of Cameroon, Plasmodium yoelii, P. chabaudi and P. vinckei. Parasitology 86: 221-229, 1983.
Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium vinckei brucechwatti 1/69

Type
Rodent malaria line
Description
Uncloned P.v. brucechwatti 1/69, after mosquito transmission.
Subclone of
Isolate 1/69
Country
Nigeria
History
Isolate collected in 1969 by Killick-Kendrick from mosquito. Mouse named 1β arrived in Edinburgh 23.7.70 -> 5x mouse passage; stabilates made 1/9/70. 3x mouse passage -> An. stephensi transmission; stabilates made 22/12/77 and 28/3/10 Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Available.
References

Beale GH, et al. Genetics In: Killick-Kendrick R, Peters W. Rodent Malaria. London: Academic Press. pp. 213-245, 1978.
Killick-Kendrick R.. Parasitic protozoa of the blood of rodents V. Plasmodium vinckei bricechwatti subsp. nov., a malaria parasite of the thicket rat, Thamnomys rutilans, in Nigeria. Ann. Parasitol. 50: 251-264, 1975. Killick-Kendrick R. Malaria parasites of Thamnomys rutilans (Rodentia, Muridae) in Nigeria. Bull World Health Organ. 1968; 38(5): 822?824
Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium yoelii subsp. EL (10EL1)

Type
Rodent malaria line
Subclone of
isolate Esekam IV (EL)
Country
Cameroon
UK Biosafety level
1
Availability
Available.
References

Lainson F.A.. Observations on the morphology and electrophoretic variation of enzymes of the rodent malaria parasites of Cameroon, Plasmodium yoelii, P. chabaudi and P. vinckei. Parasitology 86: 221-229, 1983. Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium vinckei brucechwatti N48 (DB)

Type
Rodent malaria line
Description
uncloned P.v. brucechwatti N48 after mosquito transmission.
Subclone of
Isolate N48 (DB)
Country
Nigeria
History
Isolate N48 collected in 1967. Infected mice (3rd passage from wild) sent to Edinburgh 25.10.71 by R. Killick-Kendrick, Imperial College, London. Passaged into mouse 1DB; frozen stabilates; 5/11/71. 5 mouse passages -> An. stephensi transmission; frozen stabilates made 2/8/77 and 11/12/2009. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Available.
References

Beale GH, et al. Genetics In: Killick-Kendrick R, Peters W. Rodent Malaria. London: Academic Press. pp. 213-245, 1978. Killick-Kendrick R.. Parasitic protozoa of the blood of rodents V. Plasmodium vinckei bricechwatti subsp. nov., a malaria parasite of the thicket rat, Thamnomys rutilans, in Nigeria. Ann. Parasitol. 50: 251-264, 1975.
Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium vinckei lentum 170L (DE)

Type
Rodent malaria line
Description
P.v. lentum DE. Uncloned line, no mosquito transmission.
Subclone of
Isolate 170L (DE)
Country
Brazzaville
History
Isolate collected in 1966 by I. Landau and colleagues from Thamnomys rutilans in Congo-Brazzaville. Passaged through mice, cryopreserved. Received Edinburgh from R. Killick-Kendrick, Imperial College London, 25.10.71. Passaged three additional times through mice.
UK Biosafety level
1
Availability
Enquire.

Plasmodium vinckei lentum 194zz

Type
Rodent malaria line
Description
P.v.lentum 194zz uncloned line. No mosquito transmission.
Subclone of
Isolate 194zz (DJ)
Country
Brazzaville
History
Original isolate from wild-caught T. rutlians in Congo-Brazzaville. Passaged into mouse 28.10.66. Deep freeze -> mosquito, mouse x4. Second infected mouse named 4DJ received from I Landau 24/12/71, only P.v. lentum visible, isolate 194zz (DJ). Deep froze sample 6/1/72. Refroze 16/12/9.
UK Biosafety level
1
Availability
Enquire.
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Rodent malaria line

Please state in any acknowledgements:

 

Rodent malaria line was obtained from The European Malaria Reagent Repository (http://www.malariaresearch.eu).

It is a condition of the supply of this reagent that you also cite the reference and source given above in any publication.

Contact

Email: emrr@ed.ac.uk