Rodent malaria line

Description
P.c.chabaudi CQ23 clone. No mosquito transmission.
Subclone of
Isolate CQ
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70. Passaged into G.surdaster; frozen isolates, 12/10/70. -> 3x mouse passage, cloning; frozen isolate CQ23, 4/6/98 Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
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Description
P.v. vinckei VIBA cyPL. Uncloned strain after mosquito transmission.
Subclone of
Isolate VIBA cy Pl
Country
DRC
History
Isolated by J.M. Bafort from Anopheles dureni millecampsi, 1967. Strain received from Jan Barfoot 15/11/1972 after An. stephensi transmission. Frozen 2DO, 16/11/72
UK Biosafety level
1
Availability
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Description
P.c. chabaudi CR509 and 197CR36 clones. No mosquito transmission.
Subclone of
Isolate CR
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70. Passaged into G.surdaster; frozen isolates, 9/10/70. -> 2x mouse passage, cloning; frozen stabilate CR509, 4/4/98. Or 3x mouse passage, cloning; frozen stabilate 197CR36, 29/11/73. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
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Description
P.y.killicki 193L, uncloned line, after mosquito transmission.
Subclone of
isolate 193L (265zz)
Country
Brazzaville
History
Isolated in Brazzaville by I Landau and colleagues. 314zz (DF) and 265zz (DG) stabilates received from R Killick-Kendrick 25/10/71. Passaged into mice; deep froze stabilates, 4/11/71 (1DF). -> 2x G.surdaster passage, An. stephensi transmission, 2x mouse passage; deep froze isolates (24DF) 16/9/77. Refroze, 16/2/09.
UK Biosafety level
1
Availability
Available.
Comments
not known if this is clonal
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Description
P.c chabaudi CW191 clone. After mosquito transmission.
Subclone of
Isolate CW
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70. Passaged into G.surdaster; frozen isolates, 5/10/70. passage x2 mice, cloning; frozen stabilate 7CW37, 14/4/96. 4x mouse passage, An. stephensi transmission; frozen stabilate CW191, 19/5/99 Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
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Description
P.y. killicki uncloned line. No mosquito transmission.
Subclone of
isolate 194zz
Country
Brazzaville
History
Original isolate from wild-caught T. rutlians in Congo-Brazzaville. Passaged into mouse 28.10.66. Deep freeze -> mosquito, mouse x4. Received Edinburgh from Paris 25/11/68, passaged into mice; deep froze isolates 2N1, 11/12/68, 22N2, 6/12/68. Refroze 11/12/09
UK Biosafety level
1
Availability
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Comments
may be mixed infection with P.v. lentum
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Description
P.c.chabaudi clone ER, after mosquito transmission
Subclone of
Isolate ER (56L)
Country
Central African Republic
History
Originally isolated from Thamnomys rutilans, La Maboké field station, CAR. Mice obtained from Dr Cecile Hamers-Casterman, Brussels, 20.12.83. 7x mouse passage, cloning; frozen stabilate, 11ER, 1/2/84. An stephensi transmissions; frozen stabilates 13/3/84 (23ER), 3/7/84 (97ER), 9/7/84 (105ER) Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
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Subclone of
isolate N48
Country
Nigeria
History
Blood isolates of naturally-infected Thicket Rats captured in the Cameroon in 1974 (JM Bafort) Deep-froze samples sent to Edinburgh by Marc Wery, Antwerp, 1977.
UK Biosafety level
1
Availability
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Comments
not known if this is clonal
Description
P. chabaudi subsp. EC. Uncloned, rat -adapted strain. No mosquito transmission.
Subclone of
Isolate EC
Country
Central African Republic
History
Said to be 'original strain of Landau (1965)'. Obtained by Graham Coombs, University of Kent at Canterbury, from P. Trigg, NIMR, Mill Hill, and adapted to rats. Enzyme typing shows this strain to be distinct from other P.c. chabaudi isolates. Infected rats sent to Edinburgh by Graham Coombs, 16.05.74. Rat 1EC.
UK Biosafety level
1
Availability
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References


Coombs, G.H. and Gutteridge (1975) J. Protozool. 22, 555-560.

Please state in any acknowledgements:

 

Rodent malaria line was obtained from The European Malaria Reagent Repository (http://www.malariaresearch.eu).

It is a condition of the supply of this reagent that you also cite the reference and source given above in any publication.


Contact

Email: emrr@ed.ac.uk