Rodent malaria line

Plasmodium vinckei subsp. EK

Type
Rodent malaria line
Description
P. vinckei subsp. EK. Uncloned lines, no mosquito transmission.
Subclone of
isolate Esekam III (EK)
Country
Cameroon
History
BIsolate from T. rutilans in Cameroon by JM Bafort in March, 1974. In Antwerp, passaged x3. Frozen stabilate sent to Edinburgh 10/5/78 by Marc Wery, Antwerp. Passaged into mice 3EK and 4EK, frozen stabilates 19/5/78.
UK Biosafety level
1
Availability
Enquire.

Plasmodium yoelii yoelii AK

Type
Rodent malaria line
Description
P.y. yoelii AK lines, uncloned but after rat passage. Mosquito transmission.
Subclone of
isolate AK
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Paris 15/3/69, arrived Edinburgh 19/3/69. Passaged into mice, G.surdaster; frozen isolates, 1969. -> passage 2x rats, 3x G. surdaster, An. stephensi transmission; deep froze stabilates, 49AK, 2/3/70
UK Biosafety level
1
Availability
Enquire.
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium Chabaudi Chabaudi CB Clone 50CB2

Type
Rodent malaria line
Description
P.c. chabaudi CB clone 50CB2, after mosquito transmission. Genome sequence available.
Subclone of
Isolate CB
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud). Passaged into G.surdaster; frozen isolates, 9/10/70. 5x mouse passage, An. stephensi transmission, cloning, 50CB2; frozen stabilate 24/7/79. Revived for DNA preparation and sequencing (Otto et al, 2014). Available as stabilates after infection under SPF conditions.
UK Biosafety level
1
Availability
Available.
References

A comprehensive evaluation of rodent malaria parasite genomes and gene expression.  Otto et al., BMC Biology.  2014, 12:86

Plasmodium vinckei subsp. EL clones

Type
Rodent malaria line
Description
P. vinckei subsp. 10EL1, 10EL2, 10EL9 clones. No mosquito transmission.
Subclone of
isolate Esekam IV (EL)
Country
Cameroon
History
Original isolate Esekam IV from Thamnomys species, collected in Esekam, Cameroon in 1974 by Dr J.M. Bafort. Deep freeze stabilate sent to Edinburgh by Dr M Wery in 1977. Passaged into G surdaster, 2EL, frozen 2/11/77; mouse 1EL, frozen 2/11/77. 1EL -> 3 mouse passage, cloning; frozen stabilates, 10EL1, 10EL2, 10EL9, 9/12/1977.
UK Biosafety level
1
Availability
Available.
References

Lainson F.A.. Observations on the morphology and electrophoretic variation of enzymes of the rodent malaria parasites of Cameroon, Plasmodium yoelii, P. chabaudi and P. vinckei. Parasitology 86: 221-229, 1983.

Plasmodium yoelii yoelii AR clone

Type
Rodent malaria line
Description
P.y.yoelii AR clone (56AR), after mosquito transmission
Subclone of
isolate AR
Country
Central African Republic
History
T. rutilans. Captured Boulard (RCA), arrived Edinburgh 26/3/69. Passaged into G.surdaster; frozen isolates, 1969. Passaged into rats, 2AR; deep froze stabilates; 30/5/69. -> 4 x mouse passage, An. stephensi transmission, cloning; 56AR; deep froze stabilates, 8/5/72.
UK Biosafety level
1
Availability
Available.
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium chabaudi chabaudi CB(pyr9), CB(pyr10)

Type
Rodent malaria line
Description
P.c. chabaudi CB clones CB(pyr9), CB(pyr10), after mosquito transmission.
Subclone of
Isolate CB
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70. Passaged into G.surdaster; frozen isolates, 9/10/70. 5x mouse passage, An. stephensi transmission, cloning, 50CB2; frozen stabilate 24/7/79. Pyrimethamine selection; CB(Pyr9), 25/7/80; CB(pyr10), 31/7/80. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Available.
Links
Plasmodium chabaudi isolates, lines and clones (PDF)

Plasmodium yoelii yoelii CU clone

Type
Rodent malaria line
Description
P.y. yoelii CU clone (23CU), after mosquito transmission.
Subclone of
isolate CU
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70; frozen isolates, 14/12/70. Passaged into G.surdaster; frozen isolates, 6/10/70. -> passaged through 4x mice, 1x G. surdaster, An. stephensi transmission, cloning; deep froze stabilate 23CU, 1/5/72.
UK Biosafety level
1
Availability
Available.

Plasmodium chabaudi chabaudi CE clone

Type
Rodent malaria line
Description
P.c. chabaudi CE clone 13CE11, after mosquito transmission
Subclone of
Isolate CE
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70. Passaged into G.surdaster; frozen isolates, 5/10/70. -> passage x5 mice, An stephensi transmission; cloning; frozen stabilate 13CE11, 19/12/72.
UK Biosafety level
1
Availability
Available.
Links
Molecular evolution and phylogenetics of rodent malaria parasites
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Plasmodium vinckei vinckei VIBA cyO

Type
Rodent malaria line
Description
P.v. vinckei VIBA cyO. Uncloned strain, after mosquito transmission.
Subclone of
Isolate VIBA cy O
Country
DRC
History
Isolated by J.M. Bafort from Anopheles dureni millecampsi, 1967. Strain received from Jan Barfoot 15/11/1972 after An. stephensi transmission. Frozen 2DN, 16/11/72
UK Biosafety level
1
Availability
Enquire.
Rodent malaria line

Please state in any acknowledgements:

 

Rodent malaria line was obtained from The European Malaria Reagent Repository (http://www.malariaresearch.eu).

It is a condition of the supply of this reagent that you also cite the reference and source given above in any publication.

Contact

Email: emrr@ed.ac.uk