Rodent malaria line

Description
P.c. chabaudi AT (AT26) clone. No mosquito transmission, 5 passages from wild T. rutilans.
Subclone of
Isolate AT
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 26/3/69. Passaged into G. surdaster, frozen isolates, 1969. -> 2x mouse passage, cloning 22/1/96; frozen stabilate AT22, 26/2/96. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
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Description
P.v. petteri 197CR27 clone. No mosquito transmission
Subclone of
Isolate CR
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70. Passaged into G.surdaster; frozen isolates, 9/10/70. 3x mouse passage, cloning; frozen stabilate 197CR27, 2/12/86. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Enquire.
Description
P.c. chabaudi BC clone. No mosquito transmission, 6 passages from wild T. rutilans.
Subclone of
Isolate BC
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 11/4/69. Passaged into G.surdaster; frozen isolates, 1969. -> 2x mouse transmission, cloning 14/3/96; frozen stabilates BC23/2, 25/7/96. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Enquire.
Description
P.vinckei subsp. EE. Uncloned line, after mosquito transmission.
Subclone of
isolate Biboto 18 (EE)
Country
Cameroon
History
Original isolate from T. rutilans in Cameroon, August 1973, made by JM Bafort. In Antwerp, Passaged 14x in mice -> mosquito x2, 2 further mouse passages. Sent to Edinburgh 9/11/1977 by Marc Wery, Antwerp. 1EE1 passaged x1 through mice; frozen stabilates 7/11/77. 2EE1 passaged x1 through G surdaster, frozen stabilates 9/11/77. Refroze 14/12/2009
UK Biosafety level
1
Availability
Available.
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Description
P.c. chabaudi BK (BK28, 29) clones. No mosquito transmission, 4 passages from wild T.rutilans.
Subclone of
Isolate BK
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 18/4/69. Passaged x2 into mice; frozen isolates, 8/5/1969. -> 3x mouse passage, cloning; frozen stabilates BK28, BK29 made on the 15/3/96. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Enquire.
Description
P. vinckei subsp 29EH, 31EH, 32EH, 33EH clones. No mosquito transmission.
Subclone of
isolate Esekam I (EH)
Country
Cameroon
History
Isolate from T. rutilans in Cameroon by JM Bafort in March, 1974. In Antwerp, passaged x1 in rodent. Frozen stabilate sent to Edinburgh 24.10.1977 by Marc Wery, Antwerp. Passaged into mouse 1EH, frozen stabilate 2/11/77; G surdaster 2EH, frozen stabilate 4/11/77. Further stabilate sent to Edinburgh 10.5.78 by Marc Wery, Antwerp. Passaged into mouse 17EH, frozen stabilate 31/5/78. -> cloning; frozen stabilates, 29EH, 31EH, 32EH, 33EH, 25/8/78.
UK Biosafety level
1
Availability
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Description
P.y. yoelii AD, uncloned, after rat passage
Subclone of
Isolate AD
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Paris 15/3/69, arrived Edinburgh 19/3/69. Frozen isolate. Passaged into G.surdaster and rat, frozen isolates, 24/12/1970. 6. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Enquire.
Description
P.c. chabaudi BS clone (4BS20). No mosquito transmission, 4 passages from wild T. rutilans.
Subclone of
Isolate BS
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Edinburgh 25/9/70; frozen isolates, 16/12/70. -> passage x2 mice; cloning; frozen stabilate 4BS20, 5/12/73. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Available.
References

Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Please state in any acknowledgements:

 

Rodent malaria line was obtained from The European Malaria Reagent Repository (http://www.malariaresearch.eu).

It is a condition of the supply of this reagent that you also cite the reference and source given above in any publication.


Contact

Email: emrr@ed.ac.uk