Rodent malaria line

Plasmodium chabaudi chabaudi AS-PYR1

Type
Rodent malaria line
Subclone of
isolate AS
Country
Central African Republic
UK Biosafety level
1
Availability
Available.
Comments
pyrimethamine resistant. Whole genome sequence available
Links
Drug resistant clones AS lineage - 1 outline, nomenclature, general info.pdf
Drug resistant clones AS lineage - 2 published papers.pdf
Drug resistant clones AS lineage - 3 clone information.pdf
References

Hayton, K., Ranford-Cartwright, L.C. and Walliker, D. (2002). Sulfadoxine-pyrimethamine resistance in the rodent malaria parasite Plasmodium chabaudi.

Plasmodium chabaudi adami DK clone (19DK1)

Type
Rodent malaria line
Description
P.c. adami DK clone (19DK1 and 19DK23), after mosquito transmission. Genome sequence of 19DK1 available.
Subclone of
Isolate 556KA
Country
Brazzaville
History
Isolated Congo-Brazzaville from Thamnomys rutilans 556KA. 2 mouse passages, deep-freeze, 4 mouse passages. Mice sent to Edinburgh 04.12.71; 1DK. -> 3x mouse passage, An. stephensi transmission; frozen stabilates, 20/11/73. Cloning 28/5/74; frozen stabilates 19DK1 and 19DK23, 10/6/74. Clone 19DK1 revived for DNA preparation and sequencing (Otto et al, 2014). Available as stabilates after infection under SPF conditions.
UK Biosafety level
1
Availability
Available.
Links
Molecular evolution and phylogenetics of rodent malaria parasites
References

Carter, R. and Walliker, D. (1976) Malaria parasites of rodents of the Congo(Brazzaville): Plasmodium chabaudi adami subsp. nov. and Plasmodium vinckei lentum. Annales de Parasitologie Humaine et Compare?e 51, 637-646.
A comprehensive evaluation of rodent malaria parasite genomes and gene expression. Otto et al., BMC Biology. 2014, 12:86

Plasmodium chabaudi chabaudi AS8793/2

Type
Rodent malaria line
Subclone of
isolate AS
Country
Central African Republic
History
Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Enquire.
References

Mackinnon, A.J. and Read, A.R. (1999). Genetic relationships between parasite virulence and transmission in the rodent malaria parasite Plasmodium chabaudi. Evolution 53, 689-703.

Plasmodium chabaudi adami DS clone (15DS12)

Type
Rodent malaria line
Description
P.c. adami DS clone 15DS12, after mosquito transmission. Genome sequence available
Subclone of
Isolate 408xz
Country
Brazzaville
History
Isolated Congo-Brazzaville, 28.10.72 from Thamnomys rutilans. Frozen blood sent to LSHTM by Irene Landau. Inoculated into mice in 28.9.73, sent to Edinburgh 18.10.73 (mouse 1DS). Cloned, 19/7/74; stabilates 15DS12. 10x mouse passage, 2x mosquito transmission; stabilates made on 2/7/82. Revived for DNA preparation and sequencing (Otto et al, 2014). Available as stabilates after infection under SPF conditions.
UK Biosafety level
1
Availability
Available.
References

Carter, R. and Walliker, D. (1976) Malaria parasites of rodents of the Congo(Brazzaville): Plasmodium chabaudi adami subsp. nov. and Plasmodium vinckei lentum. Annales de Parasitologie Humaine et Compare 51, 637-646.
A comprehensive evaluation of rodent malaria parasite genomes and gene expression. Otto et al., BMC Biology. 2014, 12:86

Plasmodium chabaudi chabaudi ASsens (12531) clone

Type
Rodent malaria line
Description
P.c. chabaudi ASsens (12531) clone, 2 passages after mosquito transmission
Subclone of
isolate AS
Country
Central African Republic
UK Biosafety level
1
Availability
Available.
Comments
Sensitive. Whole genome sequence available
Links
Drug resistant clones AS lineage - 1 outline, nomenclature, general info.pdf
Drug resistant clones AS lineage - 2 published papers.pdf
Drug resistant clones AS lineage - 3 clone information.pdf

Plasmodium chabaudi chabaudi AD clone

Type
Rodent malaria line
Description
P.c. chabaudi AD88/6 clone. No mosquito transmission, 5 passages from wild T. rutilans.
Subclone of
Isolate AD
Country
Central African Republic
History
T rutilans. Captured Boulard (RCA), arrived Paris 15/3/69, arrived Edinburgh 19/3/69. Frozen isolate. Passaged into mice, frozen isolates, 1969. Cloning 10.2.96; frozen stabilates AD86 made on 26/2/96. Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Enquire.
Links
Plasmodium chabaudi isolates, lines and clones (PDF)

Plasmodium chabaudi chabaudi ASsens (28AS11) clone

Type
Rodent malaria line
Description
P.c. chabaudi ASsens clone (28AS11), after mosquito transmission
Subclone of
Isolate AS
Country
Central African Republic
History
Blood isolates of naturally-infected Thicket Rats captured in the CAR in 1965 (Landau & Chabaud)
UK Biosafety level
1
Availability
Available.
Links
Plasmodium chabaudi isolates, lines and clones (PDF)
Molecular evolution and phylogenetics of rodent malaria parasites
References

Cravo, P.V.L., Carlton, J.M-R., Hunt, P., Bisoni, L., Padua, R.A. and Walliker, D. (2003) Genetics of mefloquine resistance in the rodent malaria parasite Plasmodium chabaudi. Antimicrobial Agents and Chemotherapy 47, 709-718. Molecular evolution and phylogenetics of rodent malaria parasites. Ramiro RS, Reece SE, Obbard DJ. BMC Evol Biol. 2012 12:219

Rodent malaria line

Please state in any acknowledgements:

 

Rodent malaria line was obtained from The European Malaria Reagent Repository (http://www.malariaresearch.eu).

It is a condition of the supply of this reagent that you also cite the reference and source given above in any publication.

Contact

Email: emrr@ed.ac.uk