Monoclonal antibody

16.3

Type
Monoclonal antibody
Isotype
IgM
Target
MSP-1 (conserved epitope)
Source
Dr. Jana McBride
History
Mouse monoclonal antibody raised by immunisation with schizont extracts
Availability
Available. Enquire.

11.3

Type
Monoclonal antibody
Isotype
IgG1
Target
Not known
Source
Dr. Jana McBride
History
Mouse monoclonal antibody raised by immunisation with schizont extracts
Availability
Available. Enquire.

5.1 (anti-EXP1)

Type
Monoclonal antibody
Description
Recognises blood stage antigen EXP-1. Will only recognise parasites with an aspartic acid (D) residue at position 136 (e.g. K1 isolate). Does not recognise parasites with Glycine at the same position (Palo Alto, HB3, 3D7)
Subclone of
Fusion 5
Isotype
IgG1
Target
EXP-1 (allele specific)
Source
Dr. Jana McBride
History
Mouse monoclonal antibody raised by immunisation with schizont extracts (K1 isolate)
UK Biosafety level
1
Availability
Available. Enquire.
References

Hall et al., (1983) Mol. Biochem Parasitol. 7: 247-265

12.1 (anti-MSP1 Block 4b)

Type
Monoclonal antibody
Description
Recognises MSP-1 Block 4, C-terminal half, with the epitope sequence 334-PLPENKKKEVEG-345. This sequence is found in the MAD20 isolate. See Miller et al., 1993 Mol. Biochem. Parasitol. 59:1-14 for details of MSP-1 sequence variation
Subclone of
Fusion 12
Isotype
IgG1
Target
MSP-1 Block 4 (MAD20)
Fusion partner
NS1
Source
Dr. Jana McBride
History
Mouse monoclonal antibody raised by immunisation with schizont extracts
UK Biosafety level
1
Availability
Available. Enquire.
References

Conway et al., (1991) Exp. Parasitol. 73:469-80

12.2 (anti-MSP1 Block 2, K1 type)

Type
Monoclonal antibody
Isotype
IgG1
Target
MSP-1 Block 2 (3D7)
Source
Dr. Jana McBride
History
Mouse monoclonal antibody raised by immunisation with schizont extracts. This monoclonal recognises serine containing amino acid repeat sequences (SAQSGA) within the Block 2 region of merozoite surface protein 1 (MSP-1) from Plasmodium falciparum (3D7).
UK Biosafety level
1
Availability
Available. Enquire.
References

Cavanagh DR, McBride JS. Antigenicity of recombinant proteins derived from Plasmodium falciparum merozoite surface protein 1. Mol Biochem Parasitol. 1997 Apr;85(2):197-211.

18.2 (anti-KAHRP)

Type
Monoclonal antibody
Description
Anti- P. falciparum Knob-Associated Histidine Rich Protein (KAHRP).
Subclone of
Fusion 18
Isotype
IgG2b
Target
P. falciparum KAHRP
Fusion partner
NS1
Source
Dr. Jana McBride
History
Balb/c mice were immunised with schizont extracts of P.falciparum and parasite -reactive hybridomas isolated by limiting dilution and IFA testing.
UK Biosafety level
1
Availability
Available. Enquire.
Links
Monoclonal antibody (mAb) 18.2 (pdf)
References

Monoclonal antibody (mAb) 18.2 (pdf)

Monoclonal antibody

Please state in any acknowledgements:

 

Monoclonal antibody was obtained from The European Malaria Reagent Repository (http://www.malariaresearch.eu).

It is a condition of the supply of this reagent that you also cite the reference and source given above in any publication.

Contact

Email: emrr@ed.ac.uk