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Monoclonal antibody 7.7, specific for the EXP-2 protein, a component of the PTEX transport complex on the parasitophorous vacuole membrane, is now available from the repository. This antibody is useful for localisation of the parasite membrane in IFAT or confocal studies (see Bullen et al., (2012). J. Biol. Chem. 287: 7871

Monoclonal antibody 7.7

Stabilates of the cloned P.c.chabaudi AJ and CB and P.c.adami DK and DS lines, recently sequenced by Thomas Otto and colleagues at the Wellcome Trust Sanger Institute, are now available.  Stabilates of these lines have been made from infections in mice maintained in pathogen-free conditions.

A comprehensive evaluation of rodent malaria parasite genomes and gene expression. Otto et al., BMC Biology. 2014, 12:86

mAb 18.2, which recognises the Knob Associated Histidine Rich protein (KAHRP) is now available. PDF linked below to view technical data on the monoclonal antibody. 

New monoclonal antibodies to the Plasmodium falciparum FIKK4.2 kinase are now available. A recent gift from Dr. Odile Mercereau-Puijalon, these antibodies recognise the R45 antigen, now known as FIKK4.2. See Kats et al., (2014)  International Journal for Parasitology 44: 319–328.    

Dr. Colin Sutherland, LSHTM, UK, has deposited with the EMRR seven new culture-adapted Plasmodium falciparum lines from UK travellers. These are labelled HL1204 to HL1214 in the Plasmodium falciparum section of the reagents lists.  All are polyclonal isolates of varying drug sensitivities, from five different African countries. We have expanded all of these lines.  Vials of parasites can be shipped via our couriers worldwide. See the reagent infomation pages and the linked article below for more information on these lines.

Mike Blackman, NIMR, London, has kindly deposited  the  Plasmodium falciparum clone 1G5DiCre  for DiCre-mediated gene knock-down and selectable marker recycling. This is a novel knockout system which can produce constitutively DiCre-expressing P. falciparum clones that have broad utility for the functional analysis of essential asexual blood-stage parasite genes. The plasmid pHH1-SERA5del3preDiCre is also available. 

Anti-GST (S. japonicum) is now available - a monoclonal antibody produced as a by-product of immunisation with P. falciparum GST fusion proteins, this is a useful reagent for detection of GST fusion proteins (WB, ELISA) produced using the pGEX (™) system. Cross-reactivity with P. falciparum GST unknown.

Three monoclonal antibodies to Plasmodium falciparum glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are now available as protein G purified reagents. mAbs to GAPDH are commonly used as a loading control for Western blots, and as a marker of the parasite cytoplasm. GAPDH is a highly abundant enzyme involved in the sixth step of glycolysis. Positive in IFA with P. vivax. May also be cross-reactive with GAPDH from rodent Plasmodia.

mAb 5.1 is now available as Protein G purified antibody. 5.1 recognises EXP-1.

mAb 5.1

For original descriptions see:

Nature 1984 Hope.pdf

EMBO J. 1987 Simmons.pdf

The European Malaria Reagent Repository launches its new website on 12/05/2013

The repository has a collection of parasite strains, clones and lines from both human and rodents, monoclonal antibodies to a wide range of blood stage asexual and gametocyte antigens, and a panel of ~60 recombinant antigens derived from blood stage expressed P. falciparum and P. chabaudi genes. We can also provide P. falciparum parasite extracts and DNA on request, as well as IFA slides.